Allergan Aesthetics, an AbbVie Company is seeking a highly motivated, hands-on experimentalist for the role of Scientist I, Protein Science within Toxin Innovation. The successful candidate will be a key member of a protein science team, supporting the generation and production of novel biotherapeutics with improved efficacy, selectivity and developability. As part of a matrixed organization, he/she/they will have a critical role in developing and executing protein expression and purification strategies using discovery approaches and scale to select, optimize, and prioritize therapeutic protein candidates for advancement.

The Scientist I is responsible for the recombinant expression and purification of early-stage discovery proteins. She/He/They will possess extensive expertise in E. coli protein expression, with practical knowledge of eukaryotic expression systems (baculovirus, mammalian). Demonstrated proficiency with AKTA FPLC systems developing, optimizing, and executing purification strategies (IMAC, IEX, SEC, HIC) to purify proteins with or without affinity tags is a requirement. The successful candidate will have expertise characterizing proteins (SDS-PAGE, native PAGE, ELISA, Western Blot). Familiarity with HPLC separation methods (RP, SEC, IEX), dynamic light scatter (DLS) and differential scanning fluorimetry (DSF) is desired. Experience in molecular biology techniques and knowledge of protein engineering strategies is beneficial. The candidate is expected to execute experiments with substantial independence. The candidate is expected to participate in discussions regarding the design of experiments and with guidance, have the ability to develop modified or new protocols and methods.

The position requires the candidate to productively interact with cross-functional and matrixed teams. He/She/They must be skilled in communicating and collaborating with discovery project team members at all grade levels and with diverse backgrounds, skill sets, and levels of expertise. The candidate will maintain a productive and inclusive collaborative laboratory environment consistent with department and company expectations. He/She/They must conduct all work activities in compliance with AbbVie internal and applicable regulatory requirements with strict adherence to safety policies and procedures.

• Proficient in recombinant protein expression in heterologous hosts (E. coli, baculovirus, mammalian).
• Skilled with AKTA FPLC purification systems.
• Demonstrated proficiency in protein separation techniques (IMAC, SEC, ion exchange, HIC).
• Experience with tagless protein purification
• Experience with protein characterization techniques including SDS-PAGE, native PAGE, ELISA and Western blot.
• Additional experience with protein characterization including HPLC, DLS, and DSF is desired.
• Experience with molecular biology techniques and knowledge of protein engineering strategies is beneficial
• Meticulous attention to detail and diligence in record keeping
• Excellent oral and written communication skills required as is experience with documentation of experimental plans, analyzing data and generating high quality technical reports.
• Highly autonomous and productive in performing laboratory research or method development, requiring only minimal direction from or interaction with supervisor
• Understand relevant scientific literature and apply theoretical framework to solving problems within scientific discipline in a timely manner.

Education and Experience

• Bachelor’s Degree or equivalent education and typically 5 years of experience, Master’s Degree or equivalent education and typically 2 years of experience.

• Possess thorough theoretical and practical understanding of Molecular Biology, Biochemistry and/or related discipline with a focus in heterologous protein expression, protein purification and characterization

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:

• The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future.

• We offer a comprehensive package of benefits including paid time off. (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees

• This job is eligible to participate in our short-term incentive programs.

Note: No amount of pay is considered to be wages or compensation until such amount is earned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that are allocable to a particular employee remains in the Company's sole and absolute discretion unless and until paid and may be modified at the Company’s sole and absolute discretion, consistent with applicable law.

AbbVie is an equal opportunity employer including disability/vets. It is AbbVie’s policy to employ qualified persons of the greatest ability without discrimination against any employee or applicant for employment because of race, color, religion, national origin, age, sex (including pregnancy), physical or mental disability, medical condition, genetic information, gender identity or expression, sexual orientation, marital status, status as a disabled veteran, recently separated veteran, Armed Forces service medal veteran or active duty wartime or campaign badge veteran or a person’s relationship or association with a protected veteran, including spouses and other family members, or any other protected group status. We will take affirmative action to employ and advance in employment qualified minorities, women, individuals with a disability, disabled veterans, recently separated veterans, Armed Forces service medal veterans or active-duty wartime or campaign badge veterans. The Affirmative Action Plan is available for viewing in the Human Resources office during regular business hours.